The procedure of artificial fertilization (IVF)

We do not prolong your journey to your long-desired goal by inefficient methods. From the very beginning we try to find the most effective solutions for you.
 
1. Hormonal Treatment Stimulating the Maturation of a Higher Amount of Eggs.
– GnRH antagonist/agonist suppresses the activity of gonadotropin created in the female body
– gonadotropin induces follicular growth and ovulation.
 
2. Control of the Stimulation Procedure
– transvaginal ultrasound (usually 3 times during stimulation)
– measurement of follicular growth
– the level of hormones in the blood is controlled – it depends on the individual drug dosage
 
3. Egg Retrieval (Follicular Aspiration)
– this is performed under anesthetics and is controlled by transvaginal ultrasound
– retrieval through the vaginal wall (34-36 hours after the last hormonal injection)
 
4. Sperm Retrieval
– this is performed on the same day as egg retrieval (the sperm retrieval can be performed also outside of our facility. It is necessary to collect a sterile container from the lab and transport it under specific conditions which you will be informed about.
– Sperm can also be obtained by suction from the epididymis (MESA) or removing the epithelium (TESE) – if sperm from a frozen sample should be used (donor or partner´s, e.g. after TESE, frozen before chemotherapy etc.), they are defrosted on the very same day the egg retrieval is performed.
 
5. Fertilization
– preparation of sperm and eggs – the classic method of fertilization, ICSI (intra cytoplasmic sperm injection), PICSI, IMSI.
 
6. Embryo Culture
– 1st day after the egg retrieval – control of fertilization (information about the number of mature and fertilized eggs)
– 3rd day after the egg retrieval- control of cleavage stage  (information about the development of your embryos – embryos should have 7 or more cells)
– We always perform extended culture until 5th or 6th day of development. This method helps us to achieve embryos of good quality and it increases your odds of getting pregnant.
 
7. PGD/PGS examination
– if you plan to have PGD/PGS examinations carried out, you should know, that it is possible to do in only on embryos of certain developmental stages – hatching blastocyst (HB), and fully hatched blastocyst (FHB), which are stages typical for the 5th and 6th day od development. After biopsy the embryos are frozen and used later in a natural cycle.
 
8. Embryo Transfer into the Uterus
– 5th day after  the egg retrieval – embryo transfer, the possibility of freezing good quality embryos
– 6th day after  the egg retrieval – embryo transfer (if not performed the on day 5), the possibility of freezing a good quality embryo; the last day of embryo culture.
 
9. Pregnancy Test
– 12 days after an embryo transfer, a pregnancy test can be carried out. If the result is negative, it is repeated two days later.

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